Estimation of Orphenadrine Citrate in Tablet Dosage Form by Rp-hplc Method

A sensitive, fast, precise, reversed phasehigh performance liquid chromatography (RP-HPLC) method is developed for the determination of orphenadrine citrate in tablet dosage form. Linearity, precision, limit of detection (LOD), robustness and ruggedness, drug recovery and the system suitability parameters have been validated for the developed method. The HPLC conditions are methanol: acetonitrile: water (40:30:30, v/v/v) mobile phase, Zodiac C18 column (250x 4.6 mm x 5 μm), pump pressure (10.6 MPa), flow rate (1.0 ml/min) and the wavelength of detection is 217 nm. The measured retention time of orphenadrine citrate is 5.35 minutes. The limit of detection is 0.3 μg/ml. The linearity range measured is from 10-70 μg/mL with a correlation coefficient (R 2 = 0.998). The above measured parameters indicate the developed method is useful in determination of orphenadrine citrate in tablet dosage forms.